![Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/protein-purification/glutathione-sepharose-hp-ff/glutathione-sepharose-hp-ff.jpg)
Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B
![Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein–protein interactions of the fused protein | Experimental & Molecular Medicine Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein–protein interactions of the fused protein | Experimental & Molecular Medicine](https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Fs12276-020-00549-9/MediaObjects/12276_2020_549_Fig2_HTML.png)
Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein–protein interactions of the fused protein | Experimental & Molecular Medicine
![Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein–protein interactions of the fused protein | Experimental & Molecular Medicine Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein–protein interactions of the fused protein | Experimental & Molecular Medicine](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs12276-020-00549-9/MediaObjects/12276_2020_549_Fig1_HTML.png)
Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein–protein interactions of the fused protein | Experimental & Molecular Medicine
![pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli | Nature Protocols pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli | Nature Protocols](https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Fnprot.2006.310/MediaObjects/41596_2006_Article_BFnprot2006310_Fig2_HTML.jpg)
pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli | Nature Protocols
![GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins](https://app.jove.com/files/ftp_upload/50320/50320fig1highres.jpg)
GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
![PPT - Detection of Protein-Protein Interactions Using the GST Fusion Protein Pull-down Technique PowerPoint Presentation - ID:6521621 PPT - Detection of Protein-Protein Interactions Using the GST Fusion Protein Pull-down Technique PowerPoint Presentation - ID:6521621](https://image3.slideserve.com/6521621/slide4-l.jpg)